Human CXCL11/I-TAC ELISA Kit PicoKine®, Carrier-free

About this product

Human CXCL11/I-TAC ELISA Kit PicoKine® (96 Tests). Quantitate Human CXCL11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0737-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9). By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>107</td><td>122</td><td>102</td><td>116</td><td>111</td><td>7.75</td><td>6.9%</td></tr><tr><td>Sample 2</td><td>712</td><td>667</td><td>661</td><td>672</td><td>678</td><td>20.01</td><td>2.9%</td></tr><tr><td>Sample 3</td><td>1603</td><td>1507</td><td>1537</td><td>1543</td><td>1547</td><td>34.82</td><td>2.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-X-C motif chemokine 11;Beta-R1;H174;Interferon gamma-inducible protein 9;IP-9;Interferon-inducible T-cell alpha chemoattractant;I-TAC;Small-inducible cytokine B11;CXCL11;ITAC, SCYB11, SCYB9B;

Gene Name

C-X-C motif chemokine 11

Gene ID

6373

UniProt

O14625

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F22-F94

Tissue Specificity

High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10365 MW

Specificity

Natural and recombinant human CXCL11

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Protein Name

C-X-C motif chemokine 11