Human MIF ELISA Kit PicoKine®, Carrier-free

About this product

Human MIF ELISA Kit PicoKine® (96 Tests). Quantitate Human MIF in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0813-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Macrophage migration inhibitory factor (MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>280</td><td>266</td><td>256</td><td>273</td><td>268</td><td>8.87</td><td>3.3%</td></tr><tr><td>Sample 2</td><td>1655</td><td>1562</td><td>1783</td><td>1751</td><td>1687</td><td>86.54</td><td>5.1%</td></tr><tr><td>Sample 3</td><td>4559</td><td>4531</td><td>3899</td><td>4612</td><td>4400</td><td>290.85</td><td>6.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Macrophage migration inhibitory factor ;MIF ;

Gene Name

macrophage migration inhibitory factor (glycosylation-inhibiting factor)

Gene ID

4282

UniProt

I4AY87

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P2-A115

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<20 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

12476 MW

Specificity

Natural and recombinant human MIF

Anticoagulant

heparin or EDTA

Protein Name

Macrophage migration inhibitory factor