Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine®, Carrier-free

About this product

Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine® (96 Tests). Quantitate Human MST1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0814-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Macrophage Stimulating 1 (SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>250</td><td>301</td><td>271</td><td>291</td><td>278</td><td>19.56</td><td>7%</td></tr><tr><td>Sample 2</td><td>1105</td><td>1177</td><td>1061</td><td>1161</td><td>1126</td><td>46.07</td><td>4%</td></tr><tr><td>Sample 3</td><td>6318</td><td>6544</td><td>5798</td><td>6359</td><td>6254</td><td>277.1</td><td>4.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Hepatocyte growth factor-like protein;Macrophage stimulatory protein;Macrophage-stimulating protein;MSP;Hepatocyte growth factor-like protein alpha chain;Hepatocyte growth factor-like protein beta chain;MST1;D3F15S2, DNF15S2, HGFL;

Gene Name

Hepatocyte growth factor-like protein

Gene ID

4485

UniProt

P26927

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-G711

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

80320 MW

Specificity

Natural and recombinant human MSP

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein Name

Hepatocyte growth factor-like protein