- Catalog number
- EK0825-carrier-free
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Background
- Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a protein that in humans is encoded by the OLR1 gene. It belongs to the C-type lectin superfamily. The LOX1 gene is mapped to to 12p13-p12. The protein has got the amino acids of 363. It can be detected in various tissues, highly expressed in placenta. This protein may be involved in the regulation of Fas-induced apoptosis and play a role as a scavenger receptor. The standards of this kit are recombinant mouse LOX-1 (R60-I363), with molecular weight of 36.3kDa.
- Reproducibility
- <table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>95</td><td>102</td><td>95</td><td>104</td><td>99</td><td>4.06</td><td>4.1%</td></tr><tr><td>Sample 2</td><td>321</td><td>304</td><td>284</td><td>296</td><td>301</td><td>13.44</td><td>4.4%</td></tr><tr><td>Sample 3</td><td>767</td><td>794</td><td>819</td><td>788</td><td>792</td><td>18.53</td><td>2.3%</td></tr></table>*number of samples for each test n=16.
- Synonyms
- Oxidized low-density lipoprotein receptor 1;Ox-LDL receptor 1;Lectin-like oxidized LDL receptor 1;LOX-1;Lectin-like oxLDL receptor 1;Lectin-type oxidized LDL receptor 1;Oxidized low-density lipoprotein receptor 1, soluble form;Olr1;Lox1;
- Gene Name
- Oxidized low-density lipoprotein receptor 1
- Gene ID
- 108078
- UniProt
- Q9EQ09
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: R60-I363
- Applications
- ELISA
- Standard
- 10 ng/tube
- Sample Type
- cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
- Detection Range
- 31.2 pg/ml - 2,000 pg/ml
- Sensitivity
- <1 pg/ml
- Function
- Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria (By similarity). .
- Components
- Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
- Storage Conditions
- Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 41643 MW
- Specificity
- Natural and recombinant mouse LOX-1
- Anticoagulant
- heparin or EDTA
- Subcellular Location
- Cell membrane ; Lipid-anchor . Cell membrane ; Single-pass type II membrane protein . Membrane raft . Secreted . A secreted form also exists. Localization to membrane rafts requires palmitoylation (By similarity). .
- Sequence Similarities
- Contains 1 C-type lectin domain.
- Protein Name
- Oxidized low-density lipoprotein receptor 1
