Human Nidogen-1 / Entactin / NID-1 / NID1 ELISA Kit PicoKine®, Carrier-free

About this product

Human Nidogen-1 / Entactin / NID-1 / NID1 ELISA Kit PicoKine® (96 Tests). Quantitate Human NID1 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0856-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Nidogen-1 (NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans (heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it (along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>132</td><td>145</td><td>147</td><td>147</td><td>142</td><td>6.25</td><td>4.4%</td></tr><tr><td>Sample 2</td><td>391</td><td>380</td><td>383</td><td>427</td><td>395</td><td>18.76</td><td>4.7%</td></tr><tr><td>Sample 3</td><td>1350</td><td>1536</td><td>1504</td><td>1575</td><td>1491</td><td>85.33</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Nidogen-1;NID-1;Entactin;NID1;NID;

Gene Name

Nidogen-1

Gene ID

4811

UniProt

P14543

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-K1114

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

136377 MW

Specificity

Natural and recombinant human Nidogen-1

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Sequence Similarities

Contains 6 EGF-like domains.

Protein Name

Nidogen-1