Human PAI-1 ELISA Kit PicoKine®, Carrier-free

About this product

Human PAI-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINE1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0859-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>203</td><td>218</td><td>218</td><td>234</td><td>218</td><td>10.96</td><td>5%</td></tr><tr><td>Sample 2</td><td>1208</td><td>1308</td><td>1324</td><td>1403</td><td>1310</td><td>69.37</td><td>5.2%</td></tr><tr><td>Sample 3</td><td>5496</td><td>5184</td><td>6077</td><td>5696</td><td>5613</td><td>324</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Plasminogen activator inhibitor 1;PAI;PAI-1;Endothelial plasminogen activator inhibitor;Serpin E1;SERPINE1;PAI1, PLANH1;

Gene Name

Plasminogen activator inhibitor 1

Gene ID

5054

UniProt

P05121

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-P402

Tissue Specificity

Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate ).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

45060 MW

Specificity

Natural and recombinant human PAI-1

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the serpin family.

Protein Name

Plasminogen activator inhibitor 1