Human BMP-7 ELISA Kit PicoKine®, Carrier-free

About this product

Human BMP-7 ELISA Kit PicoKine® (96 Tests). Quantitate Human BMP7 in bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0884-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>56</td><td>49</td><td>57</td><td>56</td><td>54</td><td>3.2</td><td>5.9%</td></tr><tr><td>Sample 2</td><td>263</td><td>286</td><td>255</td><td>285</td><td>272</td><td>13.55</td><td>4.9%</td></tr><tr><td>Sample 3</td><td>982</td><td>921</td><td>916</td><td>948</td><td>941</td><td>26.23</td><td>2.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Bone morphogenetic protein 7 (Osteogenic protein 1), isoform CRA_b ;cDNA FLJ78019, highly similar to Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1), mRNA ;cDNA, FLJ92758, Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1) (BMP7), mRNA ;BMP7 ;hCG_40100 ;

Gene Name

bone morphogenetic protein 7

Gene ID

655

UniProt

A8K571

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S293-H431

Applications

ELISA

Standard

10 ng/tube

Sample Type

bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

49313 MW

Specificity

Natural and recombinant human BMP-7

Anticoagulant

heparin or EDTA

Protein Name

Bone morphogenetic protein 7