Rat MCP-1 ELISA Kit PicoKine®, Carrier-free

About this product

Rat MCP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat CCL2 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0902-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>41</td><td>37</td><td>39</td><td>42</td><td>39</td><td>1.92</td><td>4.9%</td></tr><tr><td>Sample 2</td><td>257</td><td>231</td><td>216</td><td>228</td><td>233</td><td>14.94</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>473</td><td>462</td><td>446</td><td>461</td><td>460</td><td>9.6</td><td>2%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine 2;Immediate-early serum-responsive protein JE;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;MCP-1;Small-inducible cytokine A2;CCL2;Je, Mcp1, Scya2;

Gene Name

C-C motif chemokine 2

Gene ID

24770

UniProt

P14844

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-N148

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Chemotactic factor that attracts monocytes, but not neutrophils.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles. (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

16460 MW

Specificity

Natural and recombinant rat MCP-1

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 2