Human IL-31/Interleukin-31 ELISA Kit PicoKine®, Carrier-free

About this product

Human IL-31 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL31 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0979-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. This gene is mapped to 12q24.31. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL31 may function in skin immunity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>134</td><td>122</td><td>129</td><td>116</td><td>125</td><td>6.83</td><td>5.4%</td></tr><tr><td>Sample 2</td><td>804</td><td>765</td><td>827</td><td>741</td><td>784</td><td>33.38</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>1907</td><td>1624</td><td>1623</td><td>1803</td><td>1739</td><td>121.45</td><td>6.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-31;IL-31;IL31;

Gene Name

Interleukin-31

Gene ID

386653

UniProt

Q6EBC2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-T164

Tissue Specificity

Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

18205 MW

Specificity

Natural and recombinant human IL-31

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Protein Name

Interleukin-31