Mouse TGF-Beta 2 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse TGF-Beta 2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tgfb2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0983-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>98</td><td>90</td><td>99</td><td>91</td><td>94</td><td>4.03</td><td>4.2%</td></tr><tr><td>Sample 2</td><td>235</td><td>221</td><td>227</td><td>238</td><td>230</td><td>6.68</td><td>2.9%</td></tr><tr><td>Sample 3</td><td>827</td><td>847</td><td>843</td><td>932</td><td>862</td><td>40.95</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Transforming growth factor beta-2;TGF-beta-2;Latency-associated peptide;LAP;Tgfb2;

Gene Name

Transforming growth factor beta-2

Gene ID

21808

UniProt

P27090

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A303-S414

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

47589 MW

Specificity

Natural and recombinant mouse TGF-beta 2

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted.

Protein Name

Transforming growth factor beta-2