Rat TGF-Beta 3 ELISA Kit PicoKine®, Carrier-free

About this product

Rat TGF-Beta 3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Tgfb3 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1105-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>77</td><td>75</td><td>69</td><td>73</td><td>73</td><td>2.95</td><td>4%</td></tr><tr><td>Sample 2</td><td>234</td><td>208</td><td>238</td><td>199</td><td>219</td><td>16.61</td><td>7.5%</td></tr><tr><td>Sample 3</td><td>841</td><td>937</td><td>936</td><td>884</td><td>899</td><td>40</td><td>4.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Transforming growth factor beta-3;TGF-beta-3;Latency-associated peptide;LAP;Tgfb3;Tgf-b3;

Gene Name

Transforming growth factor beta-3

Gene ID

25717

UniProt

Q07258

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A301-S412

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (EDTA) and urine.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Involved in embryogenesis and cell differentiation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

47116 MW

Specificity

Natural and recombinant rat TGF-beta 3

Anticoagulant

EDTA

Subcellular Location

Secreted.

Protein Name

Transforming growth factor beta-3