Human Granzyme B ELISA Kit PicoKine®, Carrier-free

About this product

Human Granzyme B ELISA Kit PicoKine® (96 Tests). Quantitate Human GZMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1114-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>35</td><td>36</td><td>36</td><td>35</td><td>35</td><td>0.5</td><td>1.4%</td></tr><tr><td>Sample 2</td><td>170</td><td>190</td><td>185</td><td>175</td><td>180</td><td>7.9</td><td>4.3%</td></tr><tr><td>Sample 3</td><td>476</td><td>469</td><td>464</td><td>420</td><td>457</td><td>21.92</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Granzyme B;3.4.21.79;C11;CTLA-1;Cathepsin G-like 1;CTSGL1;Cytotoxic T-lymphocyte proteinase 2;Lymphocyte protease;Fragmentin-2;Granzyme-2;Human lymphocyte protein;HLP;SECT;T-cell serine protease 1-3E;GZMB;CGL1, CSPB, CTLA1, GRB;

Gene Name

Granzyme B

Gene ID

3002

UniProt

P10144

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I21-Y247

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27716 MW

Specificity

Natural and recombinant human Granzyme B

Anticoagulant

heparin or EDTA

Subcellular Location

Cytoplasmic granule . Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

Sequence Similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Protein Name

Granzyme B