Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tlr2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1130-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282). TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>304</td><td>323</td><td>310</td><td>307</td><td>311</td><td>7.24</td><td>2.3%</td></tr><tr><td>Sample 2</td><td>1448</td><td>1379</td><td>1487</td><td>1331</td><td>1411</td><td>60.35</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>4661</td><td>5150</td><td>4522</td><td>4797</td><td>4782</td><td>233.39</td><td>4.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Toll-like receptor 2;CD282;Tlr2;

Gene Name

Toll-like receptor 2

Gene ID

24088

UniProt

Q9QUN7

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-L590

Tissue Specificity

Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

89449 MW

Specificity

Natural and recombinant mouse TLR2

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the Toll-like receptor family.

Protein Name

Toll-like receptor 2