Mouse GDF-15 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse GDF-15 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gdf15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1134-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

GDF-15 (Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by ly interfering with chemokine signaling and integrin activation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>36</td><td>32</td><td>31</td><td>30</td><td>32</td><td>2.27</td><td>7%</td></tr><tr><td>Sample 2</td><td>233</td><td>243</td><td>249</td><td>254</td><td>244</td><td>7.82</td><td>3.2%</td></tr><tr><td>Sample 3</td><td>386</td><td>396</td><td>409</td><td>421</td><td>403</td><td>13.2</td><td>3.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Growth/differentiation factor 15;GDF-15;Gdf15;Sbf;

Gene Name

Growth/differentiation factor 15

Gene ID

23886

UniProt

Q9Z0J7

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S189-A303

Tissue Specificity

Highly expressed in liver. .

Applications

ELISA

Standard

1 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

33225 MW

Specificity

Natural and recombinant mouse GDF-15

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Protein Name

Growth/differentiation factor 15