Rat FGF1/Fgf Acidic ELISA Kit PicoKine®, Carrier-free

About this product

Rat FGF1/Fgf Acidic ELISA Kit PicoKine® (96 Tests). Quantitate Rat Fgf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1173-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>101</td><td>102</td><td>89</td><td>88</td><td>95</td><td>6.51</td><td>6.8%</td></tr><tr><td>Sample 2</td><td>288</td><td>252</td><td>243</td><td>289</td><td>268</td><td>20.74</td><td>7.7%</td></tr><tr><td>Sample 3</td><td>887</td><td>811</td><td>757</td><td>818</td><td>818</td><td>46.18</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Fibroblast growth factor 1;FGF-1;Acidic fibroblast growth factor;aFGF;Heparin-binding growth factor 1;HBGF-1;Fgf1;Fgf-1, Fgfa;

Gene Name

Fibroblast growth factor 1

Gene ID

25317

UniProt

P61149

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F16-D155

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17418 MW

Specificity

Natural and recombinant rat FGF1

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted . Cytoplasm . Cytoplasm, cell cortex . Cytoplasm, cytosol . Nucleus . Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non- classical pathway that requires Cu (2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59 (By similarity). .

Protein Name

Fibroblast growth factor 1