Mouse GAS6 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse GAS6 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gas6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1196-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

GAS6 (Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid (Gla)-containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>50</td><td>58</td><td>58</td><td>56</td><td>55</td><td>3.27</td><td>5.9%</td></tr><tr><td>Sample 2</td><td>232</td><td>221</td><td>213</td><td>230</td><td>224</td><td>7.58</td><td>3.3%</td></tr><tr><td>Sample 3</td><td>1017</td><td>985</td><td>986</td><td>943</td><td>982</td><td>26.3</td><td>2.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Growth arrest-specific protein 6;GAS-6;AXL receptor tyrosine kinase ligand;Gas6;

Gene Name

Growth arrest-specific protein 6

Gene ID

14456

UniProt

Q61592

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D115-P673

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

74610 MW

Specificity

Natural and recombinant mouse GAS6

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Sequence Similarities

Contains 4 EGF-like domains.

Protein Name

Growth arrest-specific protein 6