Human MFGE8/Lactadherin ELISA Kit PicoKine®, Carrier-free

About this product

Human MFGE8/Lactadherin ELISA Kit PicoKine® (96 Tests). Quantitate Human MFGE8 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1201-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>100</td><td>98</td><td>103</td><td>96</td><td>99</td><td>2.58</td><td>2.6%</td></tr><tr><td>Sample 2</td><td>660</td><td>663</td><td>784</td><td>706</td><td>703</td><td>50.04</td><td>7.1%</td></tr><tr><td>Sample 3</td><td>1196</td><td>1025</td><td>1141</td><td>1197</td><td>1139</td><td>70.01</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Lactadherin;Breast epithelial antigen BA46;HMFG;MFGM;Milk fat globule-EGF factor 8;MFG-E8;SED1;Lactadherin short form;Medin;MFGE8;

Gene Name

Lactadherin

Gene ID

4240

UniProt

Q08431

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L24-C387

Tissue Specificity

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

43123 MW

Specificity

Natural and recombinant human MFGE8

Anticoagulant

heparin or EDTA

Subcellular Location

Membrane ; Peripheral membrane protein . Secreted .

Sequence Similarities

Contains 1 EGF-like domain.

Protein Name

Lactadherin