Human IL-18R1 ELISA Kit PicoKine®, Carrier-free

About this product

Human IL-18R1 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL18R1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1260-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The interleukin-18 receptor 1 (IL-18R1), also known as CDw218a (cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>73</td><td>73</td><td>69</td><td>77</td><td>73</td><td>2.82</td><td>3.8%</td></tr><tr><td>Sample 2</td><td>260</td><td>269</td><td>256</td><td>285</td><td>267</td><td>11.14</td><td>4.1%</td></tr><tr><td>Sample 3</td><td>905</td><td>862</td><td>955</td><td>814</td><td>884</td><td>52.12</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-18 receptor 1;IL-18R-1;IL-18R1;CD218 antigen-like family member A;CDw218a;IL1 receptor-related protein;IL-1Rrp;IL1R-rp;CD218a;IL18R1;IL1RRP;

Gene Name

Interleukin-18 receptor 1

Gene ID

8809

UniProt

Q13478

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C22-R329

Tissue Specificity

Expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression in Hodgkin disease cell lines.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor for interleukin 18 (IL-18). Binding to the agonist leads to the activation of NF-kappa-B.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

62304 MW

Specificity

Natural and recombinant human IL-18R1

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the interleukin-1 receptor family.

Protein Name

Interleukin-18 receptor 1