Human CRISP3 ELISA Kit PicoKine®, Carrier-free

About this product

Human CRISP3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CRISP3 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1284-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>110</td><td>113</td><td>130</td><td>111</td><td>116</td><td>8.15</td><td>7.0%</td></tr><tr><td>Sample 2</td><td>383</td><td>419</td><td>441</td><td>377</td><td>405</td><td>26.26</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>1477</td><td>1600</td><td>1623</td><td>1582</td><td>1570</td><td>55.90</td><td>3.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cysteine-rich secretory protein 3;CRISP-3;Specific granule protein of 28 kDa;SGP28;CRISP3;

Gene Name

Cysteine-rich secretory protein 3

Gene ID

10321

UniProt

P54108

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N21-Y245

Tissue Specificity

Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<50 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27630 MW

Specificity

Natural and recombinant human CRISP3

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted. In neutrophils, localized in specific granules.

Sequence Similarities

Belongs to the CRISP family.

Protein Name

Cysteine-rich secretory protein 3