Human CRLF2/TSLP R ELISA Kit PicoKine®, Carrier-free

About this product

Human CRLF2/TSLP R ELISA Kit PicoKine® (96 Tests). Quantitate Human CRLF2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1288-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>110</td><td>110</td><td>114</td><td>115</td><td>112</td><td>2.27</td><td>2%</td></tr><tr><td>Sample 2</td><td>661</td><td>633</td><td>642</td><td>685</td><td>655</td><td>19.92</td><td>3%</td></tr><tr><td>Sample 3</td><td>1213</td><td>1047</td><td>1166</td><td>1231</td><td>1164</td><td>71.73</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cytokine receptor-like factor 2;Cytokine receptor-like 2;IL-XR;Thymic stromal lymphopoietin protein receptor;TSLP receptor;CRLF2;CRL2, ILXR, TSLPR;

Gene Name

Cytokine receptor-like factor 2

Gene ID

64109

UniProt

Q9HC73

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G25-K231

Tissue Specificity

Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

42013 MW

Specificity

Natural and recombinant human CRLF2

Anticoagulant

heparin or EDTA

Subcellular Location

Isoform 1: Cell membrane ; Single-pass type I membrane protein .

Sequence Similarities

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein Name

Cytokine receptor-like factor 2