Rat IGFBP5 ELISA Kit PicoKine®, Carrier-free

About this product

Rat IGFBP5 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Igfbp5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1298-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>315</td><td>305</td><td>301</td><td>291</td><td>303</td><td>8.6</td><td>2.8%</td></tr><tr><td>Sample 2</td><td>1184</td><td>1112</td><td>1004</td><td>1119</td><td>1104</td><td>64.58</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>5793</td><td>5090</td><td>4974</td><td>4943</td><td>5200</td><td>346.72</td><td>6.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Insulin-like growth factor-binding protein 5;IBP-5;IGF-binding protein 5;IGFBP-5;Igfbp5;Igfbp-5;

Gene Name

Insulin-like growth factor-binding protein 5

Gene ID

25285

UniProt

P24594

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-E271

Tissue Specificity

Mostly in kidney.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30298 MW

Specificity

Natural and recombinant rat IGFBP5

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 IGFBP N-terminal domain.

Protein Name

Insulin-like growth factor-binding protein 5