Mouse CD48/Slamf2 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse CD48/Slamf2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd48 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1314-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CD48 antigen (Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>91</td><td>79</td><td>95</td><td>86</td><td>87</td><td>5.97</td><td>6.8%</td></tr><tr><td>Sample 2</td><td>816</td><td>795</td><td>773</td><td>755</td><td>784</td><td>22.93</td><td>2.9%</td></tr><tr><td>Sample 3</td><td>1332</td><td>1482</td><td>1540</td><td>1536</td><td>1472</td><td>84.28</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

CD48 antigen;BCM1 surface antigen;BLAST-1;HM48-1;MRC OX-45 surface antigen;SLAM family member 2;SLAMF2;Signaling lymphocytic activation molecule 2;sgp-60;CD48;Cd48;Bcm-1;

Gene Name

CD48 antigen

Gene ID

12506

UniProt

P18181

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F23-S217

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27383 MW

Specificity

Natural and recombinant mouse CD48

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Sequence Similarities

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Protein Name

CD48 antigen