Human Activin A ELISA Kit PicoKine®, Carrier-free

About this product

Human Activin A ELISA Kit PicoKine® (96 Tests). Quantitate Human INHBA in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0301-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>34</td><td>40</td><td>35</td><td>33</td><td>35</td><td>2.69</td><td>7.6%</td></tr><tr><td>Sample 2</td><td>199</td><td>204</td><td>220</td><td>225</td><td>212</td><td>10.79</td><td>5%</td></tr><tr><td>Sample 3</td><td>467</td><td>440</td><td>464</td><td>464</td><td>458</td><td>10.89</td><td>2.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Inhibin, beta A (Activin A, activin AB alpha polypeptide) ;Inhibin, beta A (Activin A, activin AB alpha polypeptide), isoform CRA_a ;cDNA FLJ75379, highly similar to Homo sapiens inhibin, beta A (activin A, activin AB alpha polypeptide), mRNA ;INHBA ;hCG_17267 , tcag7.474 ;

Gene Name

inhibin, beta A

Gene ID

3624

UniProt

A4D1W7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: G311-S426

Tissue Specificity

Uterus, ovary and liver.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<12 pg/ml

Function

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

47442 MW

Specificity

Natural and recombinant human Activin A

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Inhibin beta A chain