Mouse DAN/NBL1 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse DAN/NBL1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Nbl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0747-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>84</td><td>77</td><td>79</td><td>90</td><td>82</td><td>5.02</td><td>6.1%</td></tr><tr><td>Sample 2</td><td>591</td><td>589</td><td>657</td><td>668</td><td>626</td><td>36.46</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>1510</td><td>1598</td><td>1670</td><td>1616</td><td>1598</td><td>57.55</td><td>3.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Neuroblastoma suppressor of tumorigenicity 1;N03;Zinc finger protein DAN;Nbl1;Dan, Dana;

Gene Name

Neuroblastoma suppressor of tumorigenicity 1

Gene ID

17965

UniProt

Q61477

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-D178

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

19107 MW

Specificity

Natural and recombinant mouse DAN

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Protein Name

Neuroblastoma suppressor of tumorigenicity 1