Human KLK11/Kallikrein-11 ELISA Kit PicoKine®, Carrier-free

About this product

Human KLK11/Kallikrein 11 ELISA Kit PicoKine® (96 Tests). Quantitate Human KLK11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1166-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>217</td><td>206</td><td>233</td><td>215</td><td>217</td><td>9.73</td><td>4.4%</td></tr><tr><td>Sample 2</td><td>1054</td><td>1031</td><td>1100</td><td>1045</td><td>1057</td><td>25.86</td><td>2.4%</td></tr><tr><td>Sample 3</td><td>4898</td><td>4850</td><td>4518</td><td>5291</td><td>4889</td><td>274.24</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Kallikrein-11;hK11;3.4.21.-;Hippostasin;Serine protease 20;Trypsin-like protease;Kallikrein-11 inactive chain 1;Kallikrein-11 inactive chain 2;KLK11;PRSS20, TLSP;UNQ649/PRO1279;

Gene Name

Kallikrein-11

Gene ID

11012

UniProt

Q9UBX7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-N250

Tissue Specificity

Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

31059 MW

Specificity

Natural and recombinant human KLK11

Anticoagulant

heparin or EDTA

Subcellular Location

Isoform 1: Secreted.

Protein Name

Kallikrein-11