Human KLK12/Klk L5 ELISA Kit PicoKine®, Carrier-free

About this product

Human KLK12/Klk L5 ELISA Kit PicoKine® (96 Tests). Quantitate Human KLK12 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1167-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>208</td><td>206</td><td>217</td><td>216</td><td>211</td><td>4.81</td><td>2.2%</td></tr><tr><td>Sample 2</td><td>1283</td><td>1297</td><td>1371</td><td>1367</td><td>1329</td><td>39.83</td><td>2.9%</td></tr><tr><td>Sample 3</td><td>4339</td><td>4587</td><td>4086</td><td>4442</td><td>4363</td><td>182.83</td><td>4.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Kallikrein-12;3.4.21.-;Kallikrein-like protein 5;KLK-L5;KLK12;KLKL5;UNQ669/PRO1303;

Gene Name

Kallikrein-12

Gene ID

43849

UniProt

Q9UKR0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-N248

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

26734 MW

Specificity

Natural and recombinant human KLK12

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Kallikrein-12