Human Relaxin-3/RLN3 ELISA Kit PicoKine®, Carrier-free

About this product

Human Relaxin 3 ELISA Kit PicoKine® (96 Tests). Quantitate Human RLN3 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1407-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory; and likely plays a role in the generation/regulation of hippocampal theta rhythm.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>65</td><td>66</td><td>66</td><td>70</td><td>66</td><td>1.92</td><td>2.9%</td></tr><tr><td>Sample 2</td><td>225</td><td>219</td><td>209</td><td>235</td><td>222</td><td>9.43</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>942</td><td>1017</td><td>854</td><td>1030</td><td>960</td><td>70.19</td><td>7.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Relaxin-3;Insulin-like peptide INSL7;Insulin-like peptide 7;Prorelaxin H3;Relaxin-3 B chain;Relaxin-3 A chain;RLN3;INSL7, RXN3, ZINS4;UNQ6188/PRO20213;

Gene Name

Relaxin-3

Gene ID

117579

UniProt

Q8WXF3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D119-C142 (A) & A27-R53 (B)

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma ( heparin).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142).

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15451 MW

Specificity

Natural and recombinant human Relaxin 3

Anticoagulant

heparin

Subcellular Location

Secreted.

Protein Name

Relaxin-3