Human Cathepsin L ELISA Kit PicoKine®, Carrier-free

About this product

Human Cathepsin L ELISA Kit PicoKine® (96 Tests). Quantitate Human CTSL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0676-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>204</td><td>202</td><td>210</td><td>182</td><td>199</td><td>10.52</td><td>5.2%</td></tr><tr><td>Sample 2</td><td>726</td><td>748</td><td>779</td><td>754</td><td>751</td><td>18.87</td><td>2.5%</td></tr><tr><td>Sample 3</td><td>1367</td><td>1597</td><td>1607</td><td>1555</td><td>1531</td><td>96.95</td><td>6.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cathepsin L1;3.4.22.15;Cathepsin L;Major excreted protein;MEP;Cathepsin L1 heavy chain;Cathepsin L1 light chain;CTSL;CTSL1;

Gene Name

Cathepsin L1

Gene ID

1514

UniProt

P07711

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E113-V333

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Important for the overall degradation of proteins in lysosomes.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

37564 MW

Specificity

Natural and recombinant human Cathepsin L

Anticoagulant

heparin or EDTA

Subcellular Location

Lysosome.

Protein Name

Cathepsin L1