Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Xcl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0803-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>209</td><td>185</td><td>189</td><td>201</td><td>196</td><td>9.53</td><td>4.8%</td></tr><tr><td>Sample 2</td><td>665</td><td>691</td><td>673</td><td>711</td><td>685</td><td>17.72</td><td>2.5%</td></tr><tr><td>Sample 3</td><td>1909</td><td>1945</td><td>1858</td><td>1909</td><td>1905</td><td>30.98</td><td>1.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Lymphotactin;C motif chemokine 1;Cytokine SCM-1;Lymphotaxin;Small-inducible cytokine C1;Xcl1;Lptn, Ltn, Scyc1;

Gene Name

Lymphotactin

Gene ID

16963

UniProt

P47993

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V22-G114

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<5 pg/ml

Function

Chemotactic activity for lymphocytes but not for monocytes or neutrophils.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

12467 MW

Specificity

Natural and recombinant mouse XCL1

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Lymphotactin