Human AFP/Alpha-fetoprotein ELISA Kit PicoKine®, Carrier-free

About this product

Human AFP/Alpha-fetoprotein ELISA Kit PicoKine® (96 Tests). Quantitate Human AFP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0651-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>95</td><td>105</td><td>109</td><td>107</td><td>104</td><td>5.38</td><td>5.1%</td></tr><tr><td>Sample 2</td><td>643</td><td>732</td><td>682</td><td>710</td><td>691</td><td>33.25</td><td>4.8%</td></tr><tr><td>Sample 3</td><td>2190</td><td>2290</td><td>2420</td><td>2404</td><td>2326</td><td>93.15</td><td>4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;AFP;HPAFP;

Gene Name

Alpha-fetoprotein

Gene ID

174

UniProt

P02771

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R19-V609

Tissue Specificity

Plasma. Synthesized by the fetal liver and yolk sac.

Applications

ELISA

Standard

5 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<10 pg/ml

Function

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

68678 MW

Specificity

Natural and recombinant human AFP

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Alpha-fetoprotein