Mouse BMP-7 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse BMP-7 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Bmp7 in bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1443-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>82</td><td>84</td><td>93</td><td>90</td><td>87</td><td>4.43</td><td>5%</td></tr><tr><td>Sample 2</td><td>219</td><td>249</td><td>237</td><td>227</td><td>233</td><td>11.22</td><td>4.8%</td></tr><tr><td>Sample 3</td><td>964</td><td>880</td><td>981</td><td>967</td><td>948</td><td>39.78</td><td>4.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Bone morphogenetic protein 7;BMP-7;Osteogenic protein 1;OP-1;Bmp7;Bmp-7, Op1;

Gene Name

Bone morphogenetic protein 7

Gene ID

12162

UniProt

P23359

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S292-H430

Applications

ELISA

Standard

10 ng/tube

Sample Type

bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

49199 MW

Specificity

Natural and recombinant mouse BMP-7

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Protein Name

Bone morphogenetic protein 7