Human ICAM5 ELISA Kit PicoKine®, Carrier-free

About this product

Human ICAM5 ELISA Kit PicoKine® (96 Tests). Quantitate Human ICAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1529-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Intercellular adhesion molecule 5 is a protein that in humans is encoded by the ICAM5 gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>274</td><td>247</td><td>258</td><td>248</td><td>256</td><td>10.84</td><td>4.2%</td></tr><tr><td>Sample 2</td><td>1241</td><td>1262</td><td>1337</td><td>1171</td><td>1252</td><td>59.17</td><td>4.7%</td></tr><tr><td>Sample 3</td><td>4856</td><td>4750</td><td>4812</td><td>4563</td><td>4745</td><td>111.75</td><td>2.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Intercellular adhesion molecule 5;ICAM-5;Telencephalin;ICAM5;TLCN, TLN;

Gene Name

Intercellular adhesion molecule 5

Gene ID

7087

UniProt

Q9UMF0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A28-E570

Tissue Specificity

Expressed on neurons in the most rostral segment of the mammalian brain, the telencephalon.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<15 pg/ml

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

97116 MW

Specificity

Natural and recombinant human ICAM5

Anticoagulant

heparin or EDTA

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Intercellular adhesion molecule 5