Mouse Meteorin-like/METRNL ELISA Kit PicoKine®, Carrier-free

About this product

Mouse Meteorin-like/METRNL ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Metrnl in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1550-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. In mice, exogenously elevated circulating Metrnl increased whole-body energy expenditure and improved glucose tolerance following diet-induced obesity. It also induced expression of antiinflammatory genes, leading to alternative activation of macrophages in adipose tissue and to browning of white fat depots. Metrnl did not appear to activate thermogenic genes ly, but it stimulated eosinophils and macrophages to enter adipose tissue and take their specific thermogenic actions. Blockade of cytokine signaling eliminated Metrnl-induced changes in gene expression required for alternative macrophage activation and blunted expression of beige fat thermogenic and beta-oxidation genes. It is concluded that METRNL is a hormone that influences energy expenditure and thermogenic programs in adipose tissue.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>180</td><td>180</td><td>185</td><td>186</td><td>182</td><td>2.77</td><td>1.5%</td></tr><tr><td>Sample 2</td><td>664</td><td>729</td><td>691</td><td>770</td><td>713</td><td>39.96</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>977</td><td>934</td><td>1059</td><td>1080</td><td>1012</td><td>59.45</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Meteorin-like protein;Subfatin;Metrnl;

Gene Name

Meteorin-like protein

Gene ID

210029

UniProt

Q8VE43

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q46-E311

Tissue Specificity

Highly expressed in subcutaneous adipose tissue. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<12 pg/ml

Function

Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti- inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

34530 MW

Specificity

Natural and recombinant mouse Meteorin-like

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Protein Name

Meteorin-like protein