Human CHL1/L1CAM-2 ELISA Kit PicoKine®, Carrier-free

About this product

Human CHL1/L1CAM-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CHL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1576-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is a protein that in humans is encoded by the CHL1 gene. This protein is a member of the L1 gene family of neural cell adhesion molecules. By FISH, the CHL1 gene was mapped to chromosome 3p26.1. The protein however has dynamic localisation, meaning that it has not only multiple roles in the cell, but also various locations. This protein may also play a role in the growth of certain cancers.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>291</td><td>285</td><td>268</td><td>269</td><td>278</td><td>9.98</td><td>3.5%</td></tr><tr><td>Sample 2</td><td>1965</td><td>1964</td><td>2077</td><td>2085</td><td>2022</td><td>58.31</td><td>2.8%</td></tr><tr><td>Sample 3</td><td>5542</td><td>5306</td><td>5133</td><td>4837</td><td>5204</td><td>257.08</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Neural cell adhesion molecule L1-like protein;Close homolog of L1;Processed neural cell adhesion molecule L1-like protein;CHL1;CALL;

Gene Name

Neural cell adhesion molecule L1-like protein

Gene ID

10752

UniProt

O00533

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I25-Q1096

Tissue Specificity

Expressed in the fetal and adult brain as well as in Schwann cell culture. Also detected in adult peripheral tissues. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

135071 MW

Specificity

Natural and recombinant human CHL1

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Soluble forms produced by cleavage/shedding also exist. .

Protein Name

Neural cell adhesion molecule L1-like protein