Rat BMP-9 ELISA Kit PicoKine®, Carrier-free

About this product

Rat BMP-9 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Gdf2 in bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1611-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>36</td><td>36</td><td>36</td><td>38</td><td>36</td><td>0.86</td><td>2.3%</td></tr><tr><td>Sample 2</td><td>187</td><td>182</td><td>199</td><td>172</td><td>185</td><td>9.72</td><td>5.2%</td></tr><tr><td>Sample 3</td><td>414</td><td>464</td><td>457</td><td>446</td><td>445</td><td>19.14</td><td>4.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Growth differentiation factor 2 ;Similar to Growth/differentiation factor 2 (GDF-2) (Bone morphogenetic protein 9) (BMP-9) ;Gdf2 ;LOC290921 ;rCG_42312 ;

Gene Name

growth differentiation factor 2

Gene ID

290921

UniProt

M0RB87

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S320-R430

Applications

ELISA

Standard

10 ng/tube

Sample Type

bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

47978 MW

Specificity

Natural and recombinant rat BMP-9

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .