Rat IL-12(p70) ELISA Kit PicoKine®, Carrier-free

About this product

Rat IL-12(p70) ELISA Kit PicoKine® (96 Tests). Quantitate Rat IL-12(p70) in cell culture supernatants and serum. Sensitivity: 1.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1652-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant rat IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>35</td><td>31</td><td>36</td><td>34</td><td>34</td><td>1.87</td><td>5.5%</td></tr><tr><td>Sample 2</td><td>222</td><td>226</td><td>219</td><td>229</td><td>224</td><td>3.8</td><td>1.6%</td></tr><tr><td>Sample 3</td><td>502</td><td>447</td><td>478</td><td>479</td><td>476</td><td>19.55</td><td>4.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; Il12a

Gene Name

interleukin 12A

Gene ID

4405

UniProt

Q9R103

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: (M23 - S335) + (R23 - S215)

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<1.5 pg/ml

Function

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat IL-12(P70)

Anticoagulant

/

Subcellular Location

Secreted .

Protein Name

Interleukin-12 subunit alpha