Mouse SCGB3A1 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse SCGB3A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Scgb3a1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1694-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. This gene encodes a small secreted protein that may function in the inhibition of cell growth. Expression of the related gene in humans is downregulated via DNA methylation in breast cancers and other tumor types. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>37</td><td>39</td><td>42</td><td>37</td><td>38</td><td>2.04</td><td>5.3%</td></tr><tr><td>Sample 2</td><td>194</td><td>168</td><td>184</td><td>189</td><td>183</td><td>9.75</td><td>5.3%</td></tr><tr><td>Sample 3</td><td>396</td><td>433</td><td>480</td><td>419</td><td>432</td><td>30.7</td><td>7.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Secretoglobin family 3A member 1; Cytokine HIN-1; High in normal 1; Pneumo secretory protein 2; PnSP-2; Uteroglobin-related protein 2; Scgb3a1; Hin1; Pnsp2; Ugrp2

Gene Name

secretoglobin, family 3A, member 1

Gene ID

68662

UniProt

Q920D7

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F22-G104

Tissue Specificity

Highly expressed in lung, where it localizes to epithelial cells lining the trachea and bronchi (PubMed:12406855, PubMed:12175512). Expression in lung is mainly restricted to bronchi, submucosal glands of the trachea, and tracheal epithelium, with little expression in terminal bronchioles (PubMed:12406855). Expressed in uterus where it localizes to epithelial cells of the uterine glands (PubMed:12175512). Also detected in heart, stomach and small intestine (PubMed:12175512).

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<5 pg/ml

Function

Secreted cytokine-like protein. Inhibits cell growth in vitro.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SCGB3A1

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted