Rat Agrin ELISA Kit PicoKine®, Carrier-free

About this product

Rat Agrin ELISA Kit PicoKine® (96 Tests). Quantitate Rat Agrn in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1699-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the AGRN gene.This protein has nine domains homologous to protease inhibitors.It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>57</td><td>63</td><td>64</td><td>60</td><td>61</td><td>2.73</td><td>4.4%</td></tr><tr><td>Sample . 2</td><td>219</td><td>191</td><td>217</td><td>195</td><td>205</td><td>12.59</td><td>6.1%%</td></tr><tr><td>Sample 3</td><td>767</td><td>770</td><td>857</td><td>812</td><td>801</td><td>36.65</td><td>4.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Agrin; Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; Agrn; Agrin

Gene Name

Agrin

Gene ID

25592

UniProt

P25304

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A1153-P1959

Tissue Specificity

Embryonic nervous system and muscle.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and s key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha (3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat Agrin

Anticoagulant

heparin or EDTA

Subcellular Location

Cell junction, synapse