Mouse PDGF-BB ELISA Kit PicoKine®, Carrier-free

About this product

Mouse PDGF-BB ELISA Kit PicoKine® (96 Tests). Quantitate Mouse PDGF-BB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1697-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>149</td><td>172</td><td>178</td><td>169</td><td>167</td><td>10.88</td><td>6.5%</td></tr><tr><td>Sample . 2</td><td>683</td><td>696</td><td>665</td><td>663</td><td>676</td><td>13.57</td><td>2.0%%</td></tr><tr><td>Sample 3</td><td>1203</td><td>1369</td><td>1343</td><td>1427</td><td>1335</td><td>82.32</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Epidermal growth factor-like protein 7; EGF-like protein 7; Multiple epidermal growth factor-like domains protein 7; Multiple EGF-like domains protein 7; NOTCH4-like protein; Vascular endothelial statin; VE-statin; Zneu1; Egfl7; Megf7

Gene Name

PDGF-BB

Gene ID

18591

UniProt

P31240

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S82-T190

Tissue Specificity

Expressed specifically by endothelial cells of the highly vascularized organs heart, lung and kidney.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse PDGF-BB

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted, extracellular space