- Catalog number
- EK1700-carrier-free
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Background
- Platelet-derived growth factor (PDGF) is one of numerous growth factors that regulate cell growth and division.The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.
- Reproducibility
- <table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>51</td><td>49</td><td>50</td><td>50</td><td>50</td><td>0.7</td><td>1.4%</td></tr><tr><td>Sample .
2</td><td>222</td><td>183</td><td>220</td><td>190</td><td>203</td><td>17.44</td><td>8.5%%</td></tr><tr><td>Sample 3</td><td>762</td><td>921</td><td>866</td><td>889</td><td>859</td><td>59.58</td><td>6.9%</td></tr></table>*number of samples for each test n=16.
- Synonyms
- Platelet-derived growth factor D;PDGF-D;Iris-expressed growth factor;Spinal cord-derived growth factor B;SCDGF-B;PDGFD latent form;
PDGFD receptor-binding form;IEGF;SCDGFB;MSTP036; UNQ1899/PRO4345
- Gene Name
- platelet derived growth factor D
- Gene ID
- 80310
- UniProt
- Q9GZP0
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S250-R370
- Tissue Specificity
- Expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen and colon. In the kidney, expressed by the visceral epithelial cells of the glomeruli. A widespread expression is also seen in the medial smooth muscle cells of arteries and arterioles, as well as in smooth muscle cells of vasa rectae in the medullary area. Expressed in the adventitial connective tissue surrounding the suprarenal artery. In chronic obstructive nephropathy, a persistent expression is seen in glomerular visceral epithelial cells and vascular smooth muscle cells, as well as de novo expression by periglomerular interstitial cells and by some neointimal cells of atherosclerotic vessels. Expression in normal prostate is seen preferentially in the mesenchyme of the gland while expression is increased and more profuse in prostate carcinoma. Expressed in many ovarian, lung, renal and brain cancer-derived cell lines.
- Applications
- ELISA
- Standard
- 10 ng/tube
- Sample Type
- cell culture supernatants, serum and plasma (heparin, EDTA).
- Detection Range
- 31.2 pg/ml - 2,000 pg/ml
- Sensitivity
- <10 pg/ml
- Function
- Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix (By similarity).
- Components
- Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
- Storage Conditions
- Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human PDGF-DD
- Anticoagulant
- heparin or EDTA
