Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine®, Carrier-free

About this product

Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ces1d in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1815-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

This gene is mapped to 8; 8 C5. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. Enzyme that hydrolizes long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the elimination of toxic substances. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>257</td><td>282</td><td>286</td><td>273</td><td>274</td><td>11.14</td><td>4.0%</td></tr><tr><td>Sample 2</td><td>1095</td><td>1002</td><td>969</td><td>1048</td><td>1028</td><td>47.55</td><td>4.6%</td></tr><tr><td>Sample 3</td><td>4121</td><td>3854</td><td>4005</td><td>4025</td><td>4001</td><td>95.65</td><td>2.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Carboxylesterase 1D; Carboxylesterase 3; Fatty acid ethyl ester synthase; FAEE synthase; Triacylglycerol hydrolase; TGH; Ces1d; Ces1; Ces3

Gene Name

carboxylesterase 1D

Gene ID

104158

UniProt

Q8VCT4

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y19-L565

Tissue Specificity

Highest expression occurs in liver with lower levels in adipose tissue, kidney, heart, intestine, lung, testis and thymus.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decre

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CES3

Anticoagulant

heparin or EDTA

Subcellular Location

Endoplasmic reticulum lumen, cytosol, Lipid droplet