Human JAM-B/JAM2 ELISA Kit PicoKine®, Carrier-free

About this product

Human JAM-B/JAM2 ELISA Kit PicoKine® (96 Tests). Quantitate Human JAM2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1842-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Junctional adhesion molecule B is a protein that in humans is encoded by the JAM2 gene. It is mapped to 21q21.3. This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>27</td><td>30</td><td>28</td><td>30</td><td>28</td><td>1.29</td><td>4.6%</td></tr><tr><td>Sample 2</td><td>190</td><td>212</td><td>215</td><td>192</td><td>202</td><td>11.32</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>408</td><td>430</td><td>389</td><td>450</td><td>419</td><td>22.92</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Junctional adhesion molecule B; JAM-B; Junctional adhesion molecule 2; JAM-2; Vascular endothelial junction-associated molecule; VE-JAM; CD322; JAM2; C21orf43; VEJAM; UNQ219/PRO245

Gene Name

junctional adhesion molecule 2

Gene ID

58494

UniProt

P57087

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F29-S238

Tissue Specificity

Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

May play a role in the processes of lymphocyte homing to secondary lymphoid organs.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human JAM-B/JAM2

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane, Single-pass type I membrane protein, Tight junction