Human Endoglycan/PODXL2 ELISA Kit PicoKine®, Carrier-free

About this product

Human Endoglycan/PODXL2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PODXL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1928-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Podocalyxin like 2 is a protein that in humans is encoded by the PODXL2 gene. It is mapped to 3q21.3. This gene is a member of the CD34 family of cell surface transmembrane proteins, which are characterized by an N-terminal extracellular mucin domain, globular and stalk domains, a single pass transmembrane region, and a charged cytoplasmic tail. The encoded protein is a ligand for vascular selectins.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>205</td><td>237</td><td>210</td><td>211</td><td>215</td><td>12.47</td><td>5.8%</td></tr><tr><td>Sample 2</td><td>1739</td><td>1950</td><td>1893</td><td>1973</td><td>1888</td><td>91.23</td><td>4.8%</td></tr><tr><td>Sample 3</td><td>5795</td><td>6024</td><td>5325</td><td>5519</td><td>5665</td><td>265.84</td><td>4.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Podocalyxin-like protein 2; Endoglycan; PODXL2; UNQ1861/PRO3742

Gene Name

podocalyxin like 2

Gene ID

50512

UniProt

Q9NZ53

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G33-T500

Tissue Specificity

Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Endoglycan/PODXL2

Anticoagulant

heparin or EDTA

Subcellular Location

Membrane, Single-pass type I membrane protein