Rat FLRT2 ELISA Kit PicoKine®, Carrier-free

About this product

Rat FLRT2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Flrt2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1973-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 6q31. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>53</td><td>50</td><td>55</td><td>51</td><td>52</td><td>1.92</td><td>3.6%</td></tr><tr><td>Sample 2</td><td>293</td><td>280</td><td>261</td><td>256</td><td>272</td><td>14.84</td><td>5.4%</td></tr><tr><td>Sample 3</td><td>951</td><td>833</td><td>887</td><td>863</td><td>883</td><td>43.41</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; Flrt2

Gene Name

fibronectin leucine rich transmembrane protein 2

Gene ID

299236

UniProt

D3ZTV3

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C36-T660

Tissue Specificity

Detected in brain.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells. May play a role in the migration of c

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat FLRT2

Anticoagulant

heparin or EDTA

Subcellular Location

Extracellular matrix. Secreted. Endoplasmic reticulum membrane. Microsome membrane. Cell membrane. Single-pass membrane protein. Focal adhesion. Synaptosome.