Human CD300C ELISA Kit PicoKine®, Carrier-free

About this product

Human CD300C ELISA Kit PicoKine® (96 Tests). Quantitate Human CD300C in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1981-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CMRF35-like molecule 6 (CLM-6) also known as CD300 antigen-like family member C (CD300c) is a protein that in humans is encoded by the CD300C gene. It is mapped to 17q25.1. The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>48</td><td>50</td><td>50</td><td>57</td><td>51</td><td>3.41</td><td>6.6%</td></tr><tr><td>Sample 2</td><td>226</td><td>258</td><td>264</td><td>265</td><td>253</td><td>15.95</td><td>6.3%</td></tr><tr><td>Sample 3</td><td>852</td><td>940</td><td>931</td><td>798</td><td>880</td><td>58.54</td><td>6.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

CMRF35-like molecule 6; CLM-6; CD300 antigen-like family member C; CMRF35-A; CMRF-35; Immunoglobulin superfamily member 16; IgSF16; CD300c; CMRF35; CMRF35A; CMRF35A1; IGSF16

Gene Name

CD300c molecule

Gene ID

10871

UniProt

Q08708

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G21-R183

Tissue Specificity

Present on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD300C

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane. Single-pass type I membrane protein.