Human FUT8 ELISA Kit PicoKine®, Carrier-free

About this product

Human FUT8 ELISA Kit PicoKine® (96 Tests). Quantitate Human FUT8 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1990-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Alpha- (1,6)-fucosyltransferase is an enzyme that in humans is encoded by the FUT8 gene. It is mapped to 14q23.3. This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>278</td><td>281</td><td>268</td><td>268</td><td>273</td><td>5.84</td><td>2.1%</td></tr><tr><td>Sample 2</td><td>1061</td><td>1124</td><td>1190</td><td>1141</td><td>1129</td><td>46.13</td><td>4.0%</td></tr><tr><td>Sample 3</td><td>4415</td><td>4940</td><td>4601</td><td>4553</td><td>4627</td><td>193.04</td><td>4.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Alpha- (1,6)-fucosyltransferase; Alpha1-6FucT; Fucosyltransferase 8; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; GDP-fucose--glycoprotein fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; FUT8

Gene Name

fucosyltransferase 8

Gene ID

2530

UniProt

Q9BYC5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R31-K575

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin or EDTA)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human FUT8

Anticoagulant

heparin or EDTA

Subcellular Location

Golgi stack membrane. Single-pass type II membrane protein.