Human TREM-1 ELISA Kit PicoKine®, Carrier-free

About this product

Human TREM-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human TREM1 in cell culture supernatants, lysates, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0844-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Triggering receptor expressed on myeloid cells 1 is a protein that in humans is encoded by the TREM1 gene. It is mapped to 6p21.1. This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>55</td><td>62</td><td>57</td><td>57</td><td>57</td><td>2.58</td><td>4.5%</td></tr><tr><td>Sample 2</td><td>217</td><td>241</td><td>248</td><td>252</td><td>239</td><td>13.57</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>800</td><td>870</td><td>776</td><td>899</td><td>836</td><td>50.05</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Triggering receptor expressed on myeloid cells 1; TREM-1; Triggering receptor expressed on monocytes 1; CD354; TREM1

Gene Name

triggering receptor expressed on myeloid cells 1

Gene ID

54210

UniProt

Q9NP99

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A21-R200

Tissue Specificity

Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, lysates, tissue, serum and plasma (heparin or EDTA)

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human TREM-1

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane. Single-pass type I membrane protein.