Mouse Matrilin-3 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse Matrilin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Matn3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2023-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 12 A1.1; 12 3.96 cM. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>227</td><td>212</td><td>214</td><td>224</td><td>219</td><td>6.37</td><td>2.9%</td></tr><tr><td>Sample 2</td><td>983</td><td>926</td><td>1041</td><td>1054</td><td>1001</td><td>50.88</td><td>5.0%</td></tr><tr><td>Sample 3</td><td>3812</td><td>4144</td><td>3641</td><td>3647</td><td>3811</td><td>204.13</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Matrilin-3; Matn3

Gene Name

matrilin 3

Gene ID

17182

UniProt

O35701

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A28-R481

Tissue Specificity

Strongly expressed in growing skeletal tissue such as epiphyseal growth plate or in bone undergoing growth and remodeling. In the bone, actively synthesized in osteoblasts and osteocytes. Expressed in cartilage of sternum, femur, vertebrae, trachea, articular and epiphyseal cartilage, cartilage of developing bones and bones.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Matrilin-3

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Matrilin-3