Human CLEC10A ELISA Kit PicoKine®, Carrier-free

About this product

Human CLEC10A ELISA Kit PicoKine® (96 Tests). Quantitate Human CLEC10A in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2048-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

C-type lectin domain family 10 member A also known as CLEC10A is a protein that in humans is encoded by the CLEC10A gene. This gene is mapped to 17p13.1. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>34</td><td>38</td><td>33</td><td>32</td><td>34</td><td>2.27</td><td>6.6%</td></tr><tr><td>Sample 2</td><td>244</td><td>232</td><td>243</td><td>247</td><td>241</td><td>5.67</td><td>2.3%</td></tr><tr><td>Sample 3</td><td>404</td><td>404</td><td>433</td><td>399</td><td>410</td><td>13.43</td><td>3.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-type lectin domain family 10 member A; C-type lectin superfamily member 14; Macrophage lectin 2; CD301; CLEC10A; CLECSF13; CLECSF14; HML

Gene Name

C-type lectin domain containing 10A

Gene ID

10462

UniProt

Q8IUN9

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q61-H316

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CLEC10A

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Membrane. Single-pass type II membrane protein.