Human DNER ELISA Kit PicoKine®, Carrier-free

About this product

Human DNER ELISA Kit PicoKine® (96 Tests). Quantitate Human DNER in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2080-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 2q36.3. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>75</td><td>76</td><td>117</td><td>106</td><td>94</td><td>3.65</td><td>3.9%</td></tr><tr><td>Sample 2</td><td>174</td><td>203</td><td>247</td><td>148</td><td>193</td><td>11.77</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>1710</td><td>1611</td><td>1200</td><td>1578</td><td>1525</td><td>95.6</td><td>6.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Delta and Notch-like epidermal growth factor-related receptor; DNER; BET; UNQ262; PRO299

Gene Name

delta/notch like EGF repeat containing

Gene ID

92737

UniProt

Q8NFT8

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N35-Y640

Tissue Specificity

Expressed in brain, spinal cord and adrenal gland.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<30 pg/ml

Function

Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human DNER

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Cell membrane. Single-pass type I membrane protein.